Li X B, Guo X Q
Department of Physiology, Shanghai Medical University.
Sheng Li Xue Bao. 1996 Oct;48(5):457-63.
Experiments were performed on 250-300 g male Sprague-Dawley rats under urethane (700 mg/kg) and alpha-chloralose (35 mg/kg) anesthesia. Plasma catecholamine concentration and the whole blood platelet aggregation were measured during normoxia, acute hypoxia, reoxygenation, acute hypoxia immediately after bilateral adrenalectomy and acute hypoxia at the end of 15 and 30 min after bilateral adrenalectomy. The plasma adrenaline concentration and the whole blood platelet aggregation increased markedly (P < 0.01), while the plasma noradrenaline concentration tended to increase (P > 0.05) during acute hypoxia. The plasma adrenaline concentration and the whole blood platelet aggregation are capable of restoring to the normal level during reoxygenation. The plasma noradrenaline concentration did not change, while the plasma adrenaline concentration and the whole blood platelet aggregation decreased significantly (P < 0.001 or 0.05) during acute hypoxia for 15 min immediately after bilateral adrenalectomy. On the other hand, plasma adrenaline and noradrenaline were not detectable during acute hypoxia 30 min after bilateral adrenalectomy while the whole blood platelet aggregation deceased markedly (P < 0.01). When adrenaline (1 ng/ml) was added to the blood (in vitro), the whole blood platelet aggregation increased significantly (P < 0.01). All the above results indicate that acute hypoxia leads to increament of whole blood platelet aggregation and plasma adrenaline concentration due to release from the adrenal gland and the increament of the whole blood platelet aggregation may be partly mediated by increased plasma adrenaline.
实验在体重250 - 300克的雄性Sprague - Dawley大鼠身上进行，采用氨基甲酸乙酯（700毫克/千克）和α - 氯醛糖（35毫克/千克）麻醉。在常氧、急性缺氧、复氧、双侧肾上腺切除术后立即急性缺氧以及双侧肾上腺切除术后15分钟和30分钟末急性缺氧期间，测量血浆儿茶酚胺浓度和全血血小板聚集情况。急性缺氧期间，血浆肾上腺素浓度和全血血小板聚集显著增加（P < 0.01），而血浆去甲肾上腺素浓度有增加趋势（P > 0.05）。复氧期间，血浆肾上腺素浓度和全血血小板聚集能够恢复到正常水平。双侧肾上腺切除术后立即急性缺氧15分钟时，血浆去甲肾上腺素浓度无变化，而血浆肾上腺素浓度和全血血小板聚集显著降低（P < 0.001或0.05）。另一方面，双侧肾上腺切除术后30分钟急性缺氧期间，血浆肾上腺素和去甲肾上腺素检测不到，而全血血小板聚集显著降低（P < 0.01）。当向血液中加入肾上腺素（1纳克/毫升）（体外）时，全血血小板聚集显著增加（P < 0.01）。上述所有结果表明，急性缺氧导致全血血小板聚集增加和血浆肾上腺素浓度升高，原因是肾上腺释放肾上腺素，且全血血小板聚集增加可能部分由血浆肾上腺素增加介导。
