Ni J, Zhu H, Zhang W, Wang J H, Ma Q N
Department of Physiology, Harbin Medical University.
Sheng Li Xue Bao. 1996 Oct;48(5):507-11.
It has been reported recently that IGF-II plays an important role in the regulation of ovarian function. The aim of this study is to investigate whether IGF-II can affect progesterone production by cultured rat luteal cells. The results showed that IGF-II could significantly stimulate basal progesterone production in a dose-dependent manner without altering the cAMP content. IGF-II could enhance 3H-leu incorporation into protein and 3H-TdR incorporation into DNA of the luteal cells, but these effects could be inhibited by CYX and Act D. The above results suggest that stimulating effect of IGF-II on progesterone production by rat luteal cells may be related to enhanced protein and DNA synthesis.
最近有报道称，胰岛素样生长因子-II（IGF-II）在卵巢功能调节中起重要作用。本研究的目的是探讨IGF-II是否能影响培养的大鼠黄体细胞的孕酮分泌。结果表明，IGF-II能以剂量依赖的方式显著刺激基础孕酮分泌，而不改变环磷酸腺苷（cAMP）含量。IGF-II能增强3H-亮氨酸掺入黄体细胞蛋白质和3H-胸腺嘧啶核苷掺入DNA，但这些作用可被环孢菌素（CYX）和放线菌素D（Act D）抑制。上述结果提示，IGF-II对大鼠黄体细胞孕酮分泌的刺激作用可能与蛋白质和DNA合成增强有关。
