Imamura O, Sugawara M, Furuichi Y
AGENE Research Institute, Kamakura, Japan.
Biochem Biophys Res Commun. 1997 Nov 17;240(2):335-40. doi: 10.1006/bbrc.1997.7585.
A human RNA helicase gene, DBP1, was cloned by PCR methodsusing degenerate oligonucleotide primers corresponding to highly conserved motifs among known members of the DEAH-box protein family. The full-length DBP1 contains 3028 nucleotides and codes for a protein of 813 amino acids with a calculated mol. wt. of 92723 daltons. The predicted amino acid sequence shares extensive homology with Prp2, Prp16, and Prp22 proteins, which are required to splice mRNA precursors in budding yeast. The protein encoded by DBP1 has RGD, RD, and HS(A/T) repeat motifs close to the N-terminus. Southern blot analysis suggested the presence of a homologue of the DBP1 genes in other species, and Northern blot analysis showed that DBP1 is expressed ubiquitously in various human organs investigated. The DBP1 gene was found to be on chromosome 4p15.3 and encodes a putative nuclear ATP-dependent RNA helicase.
通过聚合酶链式反应（PCR）方法，使用与DEAH框蛋白家族已知成员中高度保守基序相对应的简并寡核苷酸引物，克隆了一个人类RNA解旋酶基因DBP1。全长DBP1包含3028个核苷酸，编码一个由813个氨基酸组成的蛋白质，计算分子量为92723道尔顿。预测的氨基酸序列与酿酒酵母中剪接mRNA前体所需的Prp2、Prp16和Prp22蛋白具有广泛的同源性。DBP1编码的蛋白质在靠近N端处具有RGD、RD和HS(A/T)重复基序。Southern印迹分析表明在其他物种中存在DBP1基因的同源物，Northern印迹分析显示DBP1在所研究的各种人体器官中普遍表达。发现DBP1基因位于4号染色体p15.3上，编码一种假定的核ATP依赖性RNA解旋酶。
