Jamieson D J, Beggs J D
Institute of Cell and Molecular Biology, University of Edinburgh, UK.
Mol Microbiol. 1991 Apr;5(4):805-12. doi: 10.1111/j.1365-2958.1991.tb00753.x.
The spp81/ded1 mutations were isolated as suppressors of a Saccharomyces cerevisiae pre-mRNA splicing mutation, prp8-1. The SPP81/DED1 gene encodes a putative ATP-dependent RNA helicase. While attempting to clone the wild-type SPP81/DED1 gene we isolated plasmids which were able to suppress the cold-sensitive growth defect of spp81 mutants. These plasmids encoded a gene (named DBP1) which mapped to chromosome XVI and not to the SPP81/DED1 locus on chromosome XV. The cloned gene suppressed the defect of spp81/ded1 mutants when present on both high and low copy-number plasmids but complemented spp81/ded1 null mutants only when present on high copy-number plasmids. In contrast to the SPP81/DED1 gene the DBP1 gene was not essential for cell viability. The nucleotide sequence of the DBP1 gene revealed that it also encoded a putative ATP-dependent RNA helicase which showed considerable similarity at the amino acid level to the SPP81/DED1 protein.
spp81/ded1突变体是作为酿酒酵母前体mRNA剪接突变体prp8-1的抑制子而分离得到的。SPP81/DED1基因编码一种假定的ATP依赖性RNA解旋酶。在试图克隆野生型SPP81/DED1基因时，我们分离出了能够抑制spp81突变体冷敏感生长缺陷的质粒。这些质粒编码一个基因（命名为DBP1），该基因定位于第十六号染色体，而非第十五号染色体上的SPP81/DED1基因座。当克隆基因存在于高拷贝数和低拷贝数质粒上时，均可抑制spp81/ded1突变体的缺陷，但只有当存在于高拷贝数质粒上时，才能互补spp81/ded1缺失突变体。与SPP81/DED1基因不同，DBP1基因对细胞活力并非必需。DBP1基因的核苷酸序列显示，它也编码一种假定的ATP依赖性RNA解旋酶，该酶在氨基酸水平上与SPP81/DED1蛋白具有相当大的相似性。
