Imamura O, Saiki K, Tani T, Ohshima Y, Sugawara M, Furuichi Y
AGENE Research Institute, 200 Kajiwara, Kamakura 247-0063, Japan.
Nucleic Acids Res. 1998 May 1;26(9):2063-8. doi: 10.1093/nar/26.9.2063.
During the splicing process, spliceosomal snRNAs undergo numerous conformational rearrangements that appear to be catalyzed by proteins belonging to the DEAD/H-box superfamily of RNA helicases. We have cloned a new RNA helicase gene, designated DBP2 (DEAH-boxprotein), homologous to the Schizosaccaromyces pombe cdc28(+)/prp8(+) gene involved in pre-mRNA splicing and cell cycle progression. The full-length DBP2 contains 3400 nucleotides and codes for a protein of 1041 amino acids with a calculated mol. wt of 119 037 Da. Transfection experiments demonstrated that the GFP-DBP2 gene product, transiently expressed in HeLa cells, was localized in the nucleus. The DBP2 gene was mapped by FISH to the MHC region on human chromosome 6p21.3, a region where many malignant, genetic and autoimmune disease genes are linked. Because the expression of DBP2 gene in S.pombe prp8 mutant cells partially rescued the temperature-sensitive phenotype, we conclude that DBP2 is a functional human homolog of the fission yeast Cdc28/Prp8 protein.
在剪接过程中，剪接体snRNA会经历众多构象重排，这些重排似乎由属于RNA解旋酶DEAD/H盒超家族的蛋白质催化。我们克隆了一个新的RNA解旋酶基因，命名为DBP2（DEAH盒蛋白），它与参与前体mRNA剪接和细胞周期进程的粟酒裂殖酵母cdc28(+)/prp8(+)基因同源。全长DBP2包含3400个核苷酸，编码一个1041个氨基酸的蛋白质，计算分子量为119037道尔顿。转染实验表明，在HeLa细胞中瞬时表达的GFP-DBP2基因产物定位于细胞核。通过荧光原位杂交（FISH）将DBP2基因定位到人类染色体6p21.3的主要组织相容性复合体（MHC）区域，该区域与许多恶性、遗传和自身免疫性疾病基因相关。由于DBP2基因在粟酒裂殖酵母prp8突变细胞中的表达部分挽救了温度敏感表型，我们得出结论，DBP2是裂殖酵母Cdc28/Prp8蛋白的功能性人类同源物。
