Ruan B, Ma Y, Zhuang H
Department of Infectious Diseases, First Affiliated Hospital of Zhejiang Medical University, Hangzhou.
Zhonghua Yu Fang Yi Xue Za Zhi. 1997 Nov;31(6):352-4.
Hepatitis E virus RNA (HEV RNA) was detected in 531 serial serum specimens collected from 62 hospitalized patients with hepatitis E by reverse transcription-nested polymerase chain reaction (RT-nPCR) setting two pairs of primer from open reading frame 1 (ORF1) to understand the process of HEV infection more thoroughly. Results showed that 71 percent of the patients were positive for HEV RNA. Thirty-two of them with 288 serial serum specimens were followed up for the whole process, and 24 cases (75%) were positive for HEV RNA, positive rates declined with the course of the disease and serum HEV RNA lasted for 20.6 days in average. Serum HEV RNA was kept positive in 36 of the 44 patients (81.8%) at the time their serum alanine aminotransferase (ALT) and serum total bilirubin began to lower, and anti-HEV was detected in 39 of them (88.6%). Proportion of HEV viremia and length of it had no direct relationship with serum ALT and levels of total serum bilirubin and anti-HEV.
通过逆转录巢式聚合酶链反应（RT-nPCR），从62例戊型肝炎住院患者采集的531份连续血清标本中检测戊型肝炎病毒RNA（HEV RNA），从开放阅读框1（ORF1）设置两对引物，以更全面地了解戊型肝炎病毒感染过程。结果显示，71%的患者HEV RNA呈阳性。其中32例患者的288份连续血清标本进行了全程随访，24例（75%）HEV RNA呈阳性，阳性率随病程下降，血清HEV RNA平均持续20.6天。44例患者中，36例（81.8%）在血清丙氨酸氨基转移酶（ALT）和血清总胆红素开始下降时血清HEV RNA仍为阳性，其中39例（88.6%）检测到抗HEV。HEV病毒血症的比例及其持续时间与血清ALT、血清总胆红素水平及抗HEV无直接关系。
