Propagation and titration of murine cytomegalovirus in a continuous bone marrow-derived stromal cell line (M2-10B4).

作者信息

Lutarewych M A, Quirk M R, Kringstad B A, Li W, Verfaillie C M, Jordan M C

机构信息

Department of Medicine, University of Minnesota Medical School, Minneapolis 55455, USA.

出版信息

J Virol Methods. 1997 Nov;68(2):193-8. doi: 10.1016/s0166-0934(97)00126-2.

Murine cytomegalovirus (MCMV) can only be propagated effectively in mouse embryo fibroblast (MEF) cells. We demonstrate that MCMV replicates significantly better in M2-10B4 cells, a continuous line of murine bone marrow stromal cells. M2-10B4 cells were also comparable to MEF cells for detection of small amounts of MCMV reactivating from latently infected spleen explants. M2-10B4 cells will be very useful for studies of MCMV pathogenesis.