D'Almeida V, Hipólide D C, Azzalis L A, Lobo L L, Junqueira V B, Tufik S
Department of Biochemistry, Universidade de São Paulo, Brazil.
Neurosci Lett. 1997 Oct 10;235(1-2):25-8. doi: 10.1016/s0304-3940(97)00706-4.
Paradoxical sleep deprivation was performed on rats using platform technique to investigate the oxidative process associated with it. Levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), total glutathione (GSH) and malondialdehyde production were measured in brain of rats under control conditions (C) and those on single large platforms (SLP), multiple large platforms (MLP), single small platforms (SSP) and multiple small platforms (MSP) groups. SOD, CAT and GPx brain activity and malondialdehyde production were not modified by any of the procedures. Brain GSH, however, was significantly reduced in both SSP and SLP groups. These results suggest that paradoxical sleep deprivation per se is not associated with oxidative damage. The observed alterations could be attributed to factors such as immobilization and social isolation present in the single platform techniques.
采用平台技术对大鼠进行异相睡眠剥夺，以研究与之相关的氧化过程。在对照条件（C）下以及在单个大平台（SLP）、多个大平台（MLP）、单个小平台（SSP）和多个小平台（MSP）组的大鼠大脑中，测量超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、总谷胱甘肽（GSH）水平以及丙二醛生成量。任何一种实验程序均未改变SOD、CAT和GPx的脑活性以及丙二醛生成量。然而，SSP组和SLP组的大脑GSH均显著降低。这些结果表明，异相睡眠剥夺本身与氧化损伤无关。观察到的改变可能归因于单个平台技术中存在的诸如固定和社会隔离等因素。
