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Ca2+激活的K+通道剪接变体在雏鸡耳蜗音调轴上毛细胞之间的差异分布。

Differential distribution of Ca2+-activated K+ channel splice variants among hair cells along the tonotopic axis of the chick cochlea.

作者信息

Navaratnam D S, Bell T J, Tu T D, Cohen E L, Oberholtzer J C

机构信息

Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.

出版信息

Neuron. 1997 Nov;19(5):1077-85. doi: 10.1016/s0896-6273(00)80398-0.

Abstract

We have cloned from the receptor epithelium of the chick cochlea a family of alternatively spliced cDNAs derived from cslo, which encodes a Ca2+-activated K+ channel like those shown to help determine the resonant frequency of electrically tuned hair cells. Our results from PCRs using template RNAs from both tonotopically subdivided receptor epithelia and single hair cells demonstrate differential exon usage along the frequency axis of the epithelium at multiple splice sites in cslo. We also show that single hair cells express more than one splice variant at a given splice site. Since channel isoforms encoded by differentially spliced slo transcripts in other species are functionally heterogeneous, these data suggest that differential processing of slo transcripts may account, at least in part, for the systematic variation in hair-cell membrane properties along the frequency axis of electrically tuned auditory receptor epithelia.

摘要

我们已经从鸡耳蜗的受体上皮中克隆出了一族源自cslo的选择性剪接cDNA,cslo编码一种Ca2+激活的K+通道,类似于那些已被证明有助于确定电调谐毛细胞共振频率的通道。我们使用来自按音频拓扑细分的受体上皮和单个毛细胞的模板RNA进行PCR的结果表明,在cslo的多个剪接位点处,沿着上皮的频率轴存在外显子使用差异。我们还表明,单个毛细胞在给定的剪接位点表达不止一种剪接变体。由于其他物种中由差异剪接的slo转录本编码的通道亚型在功能上是异质的,这些数据表明,slo转录本的差异加工可能至少部分地解释了电调谐听觉受体上皮频率轴上毛细胞膜特性的系统变化。

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