Kraemer H J, Gehrke R, Breithaupt A, Breithaupt H
Department of Internal Medicine, Justus-Liebig-University, Giessen, Germany.
J Chromatogr B Biomed Sci Appl. 1997 Oct 24;700(1-2):147-53. doi: 10.1016/s0378-4347(97)00323-x.
Cefotaxime, given intravenously, is currently used as a broad-spectrum antibiotic for prophylaxis of intra- and postoperative infections in ocular lens surgery. A proposed therapeutic and economic alternative is the use of orally active fluoroquinolone ofloxacine as prophylactic agent. A HPLC method was developed for determination of both antibiotics in ocular aqueous humor and plasma in order to optimize dosage for safe surpassing minimal inhibitory concentration in the humor compartment. For plasma determinations a solid-phase extraction procedure was used with ciprofloxacine as internal standard. Detection limits for direct HPLC-analysis of ocular aqueous humor was 0.08 microg/ml for all compounds, whereas in plasma 0.31 microg/ml could be determined after solid-phase extraction.
静脉注射用头孢噻肟目前用作广谱抗生素，用于预防眼内晶状体手术的术中和术后感染。一种建议的治疗和经济替代方案是使用口服活性氟喹诺酮氧氟沙星作为预防剂。开发了一种HPLC方法，用于测定眼房水和血浆中的两种抗生素，以便优化剂量，确保在房水腔中安全超过最低抑菌浓度。对于血浆测定，使用以环丙沙星为内标的固相萃取程序。所有化合物直接HPLC分析眼房水的检测限为0.08微克/毫升，而固相萃取后血浆中可测定的检测限为0.31微克/毫升。
