Ikehira H, Obata T, Koga M, Yoshida K
Department of Radiology, Chiba University, Japan.
Acta Radiol. 1997 Nov;38(6):998-1002. doi: 10.1080/02841859709172117.
Dynamic natural-abundance 13C MR spectroscopy (MRS) studies without proton decoupling were performed in the human liver using commercial 1.5 T MR equipment.
A single tuned custom-made circular surface coil with an OD of 20 cm operating at 16.04 MHz was used for the 13C study. Seventy-five grams of glucose dissolved in water was administered for the natural-abundance 13C-MRS dynamic study which lasted for approximately 40 to 60 min. Data acquisition was broken into 20-min and 1.7-min blocks. Localized proton shimming with a whole-body coil was performed with sufficient volume to include the observing area of the surface coil; the line width of the water signal was less than 20 Hz.
The glucose and glycogen spectra were clearly visible at 80 to 120 ppm after oral administration of the glucose solution. These data demonstrate that dynamic hepatic carbohydrate metabolism can be observed with commercially available MR equipment. Given that the human hepatic glycogen pool reaches maximum level within less than 10 min, this technique should provide a direct diagnosis of hepatic carbohydrate metabolic disorders.
使用商用1.5T磁共振设备，在人体肝脏中进行不采用质子去耦的动态自然丰度13C磁共振波谱（MRS）研究。
用于13C研究的是一个外径20cm、工作频率16.04MHz的单通道定制圆形表面线圈。将75克溶于水的葡萄糖用于自然丰度13C-MRS动态研究，该研究持续约40至60分钟。数据采集分为20分钟和1.7分钟的时间段。使用全身线圈进行局部质子匀场，匀场体积足以包含表面线圈的观察区域；水信号的线宽小于20Hz。
口服葡萄糖溶液后，在80至120ppm处可清晰看到葡萄糖和糖原谱。这些数据表明，使用商用磁共振设备可以观察到肝脏碳水化合物的动态代谢。鉴于人体肝脏糖原池在不到10分钟内达到最大水平，该技术应能直接诊断肝脏碳水化合物代谢紊乱。
