Hase K, Li J, Basnet P, Xiong Q, Takamura S, Namba T, Kadota S
Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceutical University, Japan.
Chem Pharm Bull (Tokyo). 1997 Nov;45(11):1823-7. doi: 10.1248/cpb.45.1823.
The n-BuOH extract of Swertia japonica showed a significant hepatoprotective effect on D-galactosamine (D-GalN)/lipopolysaccharide (LPS)-induced liver injury in mice. The activity-guided fractionation led to the isolation of a new tetrahydroxanthone derivative, tetrahydroswertianolin (1), as well as two known iridoids, gentiopicroside (2) and sweroside (3). Their structures were elucidated by spectroscopic methods and chemical reactions. Of the three compounds, 2 and 3 possessed mild hepatoprotective activity at a dose range of 25-50 mg/kg, whereas, 1 exhibited potent activity in a dose-dependent manner. The hepatoprotective effect of tetrahydroswertianolin (1) was stronger than that of glycyrrhizin which was used as a positive control.
日本獐牙菜的正丁醇提取物对D-半乳糖胺（D-GalN）/脂多糖（LPS）诱导的小鼠肝损伤具有显著的保肝作用。通过活性导向分离，得到了一种新的四氢呫吨酮衍生物，四氢獐牙菜苦苷（1），以及两种已知的环烯醚萜苷，龙胆苦苷（2）和獐牙菜苷（3）。通过光谱方法和化学反应对它们的结构进行了阐明。在这三种化合物中，2和3在25-50 mg/kg的剂量范围内具有轻度保肝活性，而1则呈剂量依赖性地表现出强效活性。四氢獐牙菜苦苷（1）的保肝作用强于用作阳性对照的甘草酸。
