School of Pharmaceutical Sciences, Kyusyu University of Health and Welfare, 1714-1 Yoshino-cho, Nobeoka, Miyazaki, 882-8508, Japan,
J Nat Med. 2014 Jan;68(1):206-10. doi: 10.1007/s11418-013-0776-x. Epub 2013 May 8.
Aiming to examine whether the genetic background of the crude drugs derived from four Yunnanese Swertia plants and their chemical constituent profiles correlate, we analyzed the nucleotide sequences of their nuclear ribosomal DNA regions including ITS1, 5.8S ribosomal RNA gene, and ITS2, together with those of Japanese S. japonica and S. pseudochinensis from Hebei Province. The result that two of the Yunnanese Swertia plants, S. binchuanensis and S. punicea, were genetically similar may explain their similarity in chemical constituent profiles. On the other hand, in spite of differences in chemical profile, S. decora and S. pseudochinensis were genetically close. The other Yunnanese Swertia plants, S. delavayi, and S. japonica, stood at intermediate positions between these two genetically similar pairs. The result suggests that although genetic background would have an influence, environmental factors, e.g., soil and weather conditions, might be critical for their production of secondary metabolites.
为了考察来自云南四种獐牙菜属植物的生药的遗传背景与其化学成分特征是否相关，我们分析了它们的核核糖体 DNA 区（包括 ITS1、5.8S 核糖体 RNA 基因和 ITS2）的核苷酸序列，同时分析了来自河北的日本獐牙菜和假獐牙菜的序列。云南獐牙菜属的两种植物，扁茎獐牙菜和红獐牙菜，在遗传上是相似的，这一结果可能解释了它们在化学成分特征上的相似性。另一方面，尽管化学成分特征存在差异，美丽獐牙菜和假獐牙菜在遗传上是相近的。云南獐牙菜属的其他植物，华丽獐牙菜和獐牙菜，则处于这两个遗传上相似对之间的中间位置。结果表明，尽管遗传背景会产生影响，但环境因素（例如土壤和气候条件）可能对其次生代谢产物的产生具有关键作用。
