Perazzolo L M, Barracco M A
Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianópolis, Brazil.
Dev Comp Immunol. 1997 Sep-Oct;21(5):385-95. doi: 10.1016/s0145-305x(97)00022-0.
In the present study we investigated the proPO activating system of the penaeid Penaeus paulensis, focusing on its role in the shrimp immune system. The great majority of PO activity (more than 90%) was found in the shrimp hemocytes. The enzyme activity was greatly enhanced by components of microorganism cell walls, such as LPS and beta-1,3-glucans, suggesting its involvement in non-self recognition. PO activity was also found in the shrimp serum and trypsin, and LPS were able to increase the enzyme activity. Thus, serum can be used as an alternative for the study of the shrimp proPO activating system, as it is much more readily obtained than HLS. PO activity was cation-dependent, and 5 mM of calcium and 10 mM of magnesium were the optimal concentrations for the enzyme activity. An immune factor was found in the shrimp HLS, capable of inducing cell-adhesion and degranulation of the penaeid hemocytes.
在本研究中，我们调查了南美白对虾的酚氧化酶原激活系统，重点关注其在对虾免疫系统中的作用。绝大多数酚氧化酶（PO）活性（超过90%）存在于对虾血细胞中。微生物细胞壁成分，如脂多糖（LPS）和β-1,3-葡聚糖，可显著增强该酶活性，表明其参与非自我识别。在对虾血清和胰蛋白酶中也发现了PO活性，LPS能够增加该酶活性。因此，血清可作为研究对虾酚氧化酶原激活系统的替代物，因为它比血细胞更容易获得。PO活性依赖阳离子，5 mM钙和10 mM镁是该酶活性的最佳浓度。在对虾血细胞裂解物中发现了一种免疫因子，能够诱导对虾血细胞的细胞黏附和脱颗粒。
