Stambauch R, Smith M
J Exp Zool. 1976 Jul;197(1):121-5. doi: 10.1002/jez.1401970114.
Proteinase activity was found to be present along the sides of sperm penetration tunnels through the zonae pellucidae of rabbit ova using silver-proteinate staining techniques. This is the first direct confirmation of the generally accepted concept of sperm penetration by proteinase release and digestion of the zona pellucida.
利用蛋白银染色技术发现,在穿过兔卵透明带的精子穿透隧道两侧存在蛋白酶活性。这是对通过释放蛋白酶和消化透明带来实现精子穿透这一普遍接受概念的首次直接证实。