Luzza F, Oderda G, Maletta M, Imeneo M, Mesuraca L, Chioboli E, Lerro P, Guandalini S, Pallone F
Dipartimento di Medicina Sperimentale e Clinica, Università di R. Calabria, Catanzaro, Italy.
J Clin Microbiol. 1997 Dec;35(12):3358-60. doi: 10.1128/jcm.35.12.3358-3360.1997.
An in-house enzyme-linked immunosorbent assay (ELISA) for measurement of Helicobacter pylori-specific immunoglobulin G (IgG) and IgA in saliva was evaluated by comparison with histopathologic (Giemsa staining) and biochemical (urease quick test) examination of gastric biopsy specimens obtained from 112 children referred for diagnostic gastroscopy. Serum H. pylori IgG was also measured in a subgroup of 50 children by the same ELISA. Salivary H. pylori IgG levels were significantly higher in H. pylori-positive (n = 57) than in H. pylori-negative (n = 55) children (P < 0.001). The sensitivity and specificity of the salivary IgG test were 93 and 82%, respectively; the positive and negative predictive values were 84 and 92%, respectively; and the accuracy was 87.5%. Salivary H. pylori IgA did not distinguish H. pylori-positive from H. pylori-negative children. The performance of serum H. pylori IgG was slightly (3 to 6%) better than that of salivary H. pylori IgG. The salivary IgG test can be considered a useful tool for the screening of H. pylori infection in children.
通过与112名因诊断性胃镜检查而转诊儿童的胃活检标本的组织病理学（吉姆萨染色）和生化（尿素酶快速检测）检查结果进行比较，对一种用于检测唾液中幽门螺杆菌特异性免疫球蛋白G（IgG）和IgA的内部酶联免疫吸附测定（ELISA）方法进行了评估。还通过相同的ELISA法对50名儿童亚组的血清幽门螺杆菌IgG进行了检测。幽门螺杆菌阳性儿童（n = 57）的唾液幽门螺杆菌IgG水平显著高于幽门螺杆菌阴性儿童（n = 55）（P < 0.001）。唾液IgG检测的敏感性和特异性分别为93%和82%；阳性和阴性预测值分别为84%和92%；准确性为87.5%。唾液幽门螺杆菌IgA无法区分幽门螺杆菌阳性和阴性儿童。血清幽门螺杆菌IgG的性能略优于唾液幽门螺杆菌IgG（3%至6%）。唾液IgG检测可被视为筛查儿童幽门螺杆菌感染的一种有用工具。
