Orvell C, Alsheikhly A R, Kalantari M, Johansson B
Huddinge University Hospital, Department of Clinical Virology, Karolinska Institute, Sweden.
J Gen Virol. 1997 Dec;78 ( Pt 12):3187-93. doi: 10.1099/0022-1317-78-12-3187.
The SBL-1 strain of mumps virus, grouping with genotype A on the basis of the small hydrophobic protein gene sequence, was grown in the presence of three different monoclonal antibodies. The monoclonal antibodies were directed against the haemagglutinin-neuraminidase (HN) protein and they inhibited haemagglutinating activity and infectivity of the virus. The HN genes of the nine neutralization-escape virus mutants were sequenced and the predicted amino acid sequences were compared with that of the parental virus. Amino acid substitutions were found at positions 269, 352 and 354, respectively, of the 582 amino acid long protein. The three monoclonal antibodies did not react with 35 virus strains isolated in Stockholm during the years 1970 to 1985. Thirteen and four of the strains were found to belong to the D and C genotypes, respectively. A type-specific neutralization antibody response was also found in sera of rabbits hyperimmunized with purified virions of genotype A and D. The genotype-specific difference in neutralizing activity in mice and rabbits was not corroborated by an overall difference in the amino acid sequence of the HN protein of the different genotypes. Further studies are needed to explore the efficacy of mumps virus vaccines for protection against homologous and heterologous genotypes of mumps virus.
腮腺炎病毒SBL - 1株根据小疏水蛋白基因序列归类为A基因型，在三种不同单克隆抗体存在的情况下进行培养。这些单克隆抗体针对血凝素神经氨酸酶（HN）蛋白，它们抑制了病毒的血凝活性和感染性。对九个中和逃逸病毒突变体的HN基因进行了测序，并将预测的氨基酸序列与亲本病毒的序列进行了比较。在这个582个氨基酸长的蛋白中，分别在第269、352和354位发现了氨基酸替换。这三种单克隆抗体与1970年至1985年期间在斯德哥尔摩分离出的35株病毒均无反应。其中13株和4株病毒分别属于D基因型和C基因型。在用A基因型和D基因型纯化病毒粒子进行超免疫的兔血清中也发现了型特异性中和抗体反应。不同基因型的HN蛋白氨基酸序列的总体差异并未证实小鼠和兔中和活性的基因型特异性差异。需要进一步研究以探索腮腺炎病毒疫苗针对腮腺炎病毒同源和异源基因型的保护效果。
