Weiss D J, Evanson O A, McClenahan D, Fagliari J J, Jenkins K
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
Am J Vet Res. 1997 Dec;58(12):1376-80.
To determine whether platelets are hyperaggregable or form platelet-neutrophil aggregates during the prodromal stages of acute laminitis of ponies.
Healthy adult ponies: 8 experimental and 6 control.
Acute laminitis was induced by oral administration of corn starch and wood flour to 8 ponies, and indices of platelet activation were evaluated. Blood samples were collected before and at 4, 8, 12, 24, 28, and 32 hours after carbohydrate administration, and PCV, total plasma protein concentration, platelet count, activated clotting time, whole blood recalcification time, spontaneous platelet aggregation, ex vivo platelet aggregation responses, and platelet-neutrophil aggregates were determined. When lameness was first detected, ponies were euthanatized and arteriography and histologic examination of hooves were performed.
Carbohydrate overload was associated with hyperaggregability of platelets throughout the prodromal stages of laminitis and increased numbers of platelet-neutrophil aggregates. Reduction of blood supply to affected hooves was variable, and blood clots were found in 6 of 11 laminitis-affected hooves.
Platelets were hyperaggregable throughout the prodromal stages of carbohydrate-induced laminitis and formed platelet-neutrophil aggregates. Platelet-neutrophil aggregates may initiate or contribute to development of acute laminitis.
Anti-platelet therapy may be useful for treatment of acute alimentary laminitis in horses.
确定在马急性蹄叶炎前驱期血小板是否存在高聚集性或形成血小板-中性粒细胞聚集体。
健康成年马：8匹用于实验，6匹作为对照。
对8匹马口服玉米淀粉和木粉诱导急性蹄叶炎，并评估血小板活化指标。在给予碳水化合物之前以及之后4、8、12、24、28和32小时采集血样，测定血细胞比容、血浆总蛋白浓度、血小板计数、活化凝血时间、全血再钙化时间、自发性血小板聚集、体外血小板聚集反应以及血小板-中性粒细胞聚集体。首次检测到跛行时，对马实施安乐死，并进行动脉造影和蹄部组织学检查。
碳水化合物过载与蹄叶炎前驱期全程血小板高聚集性以及血小板-中性粒细胞聚集体数量增加有关。患蹄血液供应减少情况不一，在11只患蹄叶炎的蹄子中有6只发现血凝块。
在碳水化合物诱导的蹄叶炎前驱期全程血小板存在高聚集性，并形成血小板-中性粒细胞聚集体。血小板-中性粒细胞聚集体可能引发或促成急性蹄叶炎的发展。
抗血小板治疗可能对治疗马的急性营养性蹄叶炎有用。
