Kishi H, Okada T, Kawazu S, Otsuka M, Taya K, Watanabe G, Sasamoto S
Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Reprod Fertil Dev. 1997;9(4):447-53. doi: 10.1071/r96076.
To investigate the physiological importance of oestradiol-17beta and inhibin in the regulation of gonadotrophin secretion in the cyclic golden hamster, females were passively immunized against two hormones. When 200 microL antiserum against oestradiol-17beta (oestradiol-AS) was given on Day 3 (Day 1 = day of ovulation), the preovulatory gonadotrophin surge was completely blocked for 24 h and the length of the oestrous cycle was also prolonged for one day. In the group given 200 microL oestradiol-AS on Day 3, basal levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) increased slightly and superovulation (19.6+/-0.8, mean+/-s.e.m.) occurred. When 200 microL antiserum against inhibin (inhibin-AS) was given at 1100 hours on Day 3, a dramatic increase in plasma FSH and a slight increase in LH were noted, resulting in superovulation (38.2+/-2.6) on the expected Day 1. The present study indicates clearly that inhibin plays a major role in regulating the specific ovulation rate in the hamster through the control of FSH secretion. Present results also indicate that oestradiol-17beta suppresses basal LH secretion. Oestradiol-17beta may act as an indicator of the follicular maturation, and the high plasma concentration of oestradiol-17beta noted from Day 3 to Day 4 may play an important role in determining the timing of initiation of the preovulatory gonadotrophin surge.
为研究17β-雌二醇和抑制素在周期性金黄仓鼠促性腺激素分泌调节中的生理重要性，对雌性仓鼠进行了针对这两种激素的被动免疫。在第3天（第1天为排卵日）给予200μL抗17β-雌二醇抗血清（雌二醇抗血清）时，排卵前促性腺激素高峰被完全阻断24小时，发情周期长度也延长了一天。在第3天给予200μL雌二醇抗血清的组中，促卵泡激素（FSH）和促黄体生成素（LH）的基础水平略有升高，并出现了超排卵（19.6±0.8，平均值±标准误）。在第3天1100时给予200μL抗抑制素抗血清（抑制素抗血清）时，血浆FSH显著升高，LH略有升高，导致在预期的第1天出现超排卵（38.2±2.6）。本研究清楚地表明，抑制素通过控制FSH分泌在调节仓鼠的特定排卵率中起主要作用。目前的结果还表明，17β-雌二醇抑制基础LH分泌。17β-雌二醇可能作为卵泡成熟的指标，从第3天到第4天观察到的高血浆17β-雌二醇浓度可能在确定排卵前促性腺激素高峰开始的时间方面起重要作用。
