Gupta S, Schiavo D M
Lab Anim Sci. 1976 Jun;26(3):468-72.
A new and rapid method for testing low levels of ocular irritation using a balanced incomplete block design, a new grading system, and statistical analysis of the results, has been developed. Two drops (0.1 ml) of 1 of 5 ophthalmic solutions were instilled into the conjunctival sac of the right or left eye of each of 10 New Zealand white [Deb:(NZW)] rabbits hourly for 9 hr on 4 consecutive da. Before the study and before the first instillation on each day, the eyes were examined with a direct ophthalmoscope, and the reactions were graded according to a modification of the system in the illustrated Guide for Grading Eye Irritation by Hazardous Substances. The new procedure showed that 1 of the 5 solutions elicited a significantly more severe (p less than or equal to 0.05) ocular response of redness, chemosis, and discharge than did any of the other 4 solutions. Previous testing of this solution in rabbits by standard laboratory procedures and the Draize method of grading ocular irritation failed to detect these reactions.
已开发出一种新的快速方法，该方法使用平衡不完全区组设计、新的分级系统以及对结果进行统计分析来测试低水平的眼刺激。将5种眼科溶液中的1种的两滴（0.1毫升）每小时滴入10只新西兰白兔[Deb:(NZW)]每只兔子右眼或左眼的结膜囊中，连续4天，每天9小时。在研究前以及每天第一次滴入前，用直接检眼镜检查眼睛，并根据《有害物质眼刺激分级指南》图示系统的修改版对反应进行分级。新程序显示，5种溶液中的1种引起的眼红、结膜水肿和分泌物等眼部反应明显比其他4种溶液中的任何一种更严重（p小于或等于0.05）。此前通过标准实验室程序和Draize眼刺激分级方法在兔子身上对该溶液进行测试时，未能检测到这些反应。
