Kemp R B, Meredith R W, Gamble S, Frost M
Cytobios. 1983;36(143-44):153-9.
It is generally accepted that the Draize rabbit eye irritation test has several disadvantages and inadequacies when used as an indicator for potential irritancy of detergent-based commercial products. In the search for a possible in vitro screen, it was decided to use mouse LS cells in suspension, taking 50% cell death (CD50) after exposure to the product for 4 h as the endpoint. This result for eleven samples was compared with rabbit eye irritation data ranked on an arbitrary classification of mild, moderate and severe. All samples with a CD50 less than 0.5 mg ml-1 were severe eye irritants while, with one exception, all those with a CD50 greater than 1.0 mg ml-1 were mild irritants. Samples between these two cytotoxicity levels were, in general, moderately irritant to the rabbit eye. It would appear that the present in vitro test is a possible screen for the irritancy of detergent-based products.
一般认为，当用Draize兔眼刺激试验作为基于洗涤剂的商业产品潜在刺激性的指标时，该试验存在若干缺点和不足。在寻找可能的体外筛选方法时，决定使用悬浮的小鼠LS细胞，将产品暴露4小时后的50%细胞死亡（CD50）作为终点。将11个样品的该结果与根据轻度、中度和重度的任意分类排列的兔眼刺激数据进行比较。所有CD50小于0.5 mg/ml的样品都是严重的眼刺激物，而除一个例外，所有CD50大于1.0 mg/ml的样品都是轻度刺激物。处于这两种细胞毒性水平之间的样品通常对兔眼有中度刺激性。看来目前的体外试验可能是基于洗涤剂产品刺激性的一种筛选方法。
