Shimodori S, Iida K, Kojima F, Takade A, Ehara M, Amako K
School of Health Sciences, Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Microbiol Immunol. 1997;41(10):757-63. doi: 10.1111/j.1348-0421.1997.tb01923.x.
A filamentous phage was isolated from carrier strain AI-1841 of Vibrio cholerae 0139 Bengal and thus was termed fs phage. The phage was measured to be approximately 1 microm in length and 6 nm in width. One end of the phage was slightly tapered and had a fibrous appendage. The plaques developed on strain AI-4450 of V. cholerae 0139 were small and turbid. The phage grew in strain AI-4450 and reached a size of 10(8) to 10(9) pfu/ml at 5 hr after infection without inducing any lysis of the host bacteria. The group of phages attached on rod-shaped materials like fimbriae of this bacteria, with their fibrous appendages at the pointed end, were often found in the phage-infected culture. The anti-fimbrial serum effectively inhibited the infection of fs phage to the host strain AI-4450. We thus concluded that the phage can be adsorbed on fimbriae with a fibrous appendage on the pointed end of the phage filament.
从霍乱弧菌0139孟加拉型的携带菌株AI - 1841中分离出一种丝状噬菌体，因此将其命名为fs噬菌体。测得该噬菌体长度约为1微米，宽度为6纳米。噬菌体的一端略微变细，并有一个纤维状附属物。在霍乱弧菌0139的AI - 4450菌株上形成的噬菌斑小且浑浊。该噬菌体在AI - 4450菌株中生长，感染后5小时达到10⁸至10⁹噬菌斑形成单位/毫升的数量，且未诱导宿主细菌发生任何裂解。在噬菌体感染的培养物中经常发现，这类噬菌体附着在杆状物质上，如该细菌的菌毛，其纤维状附属物位于尖端。抗菌毛血清可有效抑制fs噬菌体对宿主菌株AI - 4450的感染。因此我们得出结论，该噬菌体可以通过其丝状尖端带有纤维状附属物的方式吸附在菌毛上。
