Collins V P, Brunk U T
Mech Ageing Dev. 1976 May-Jun;5(3):193-207. doi: 10.1016/0047-6374(76)90018-x.
Residual body-like structures have been shown to occur in small numbers in actively growing in vitro cultivated phase II human glia cells. In contact inhibited phase II glia cells they have been shown to accumulate. Whether such structures are analogous to lipofuscin pigment granules in post-mitotic cells in vivo has been uncertain. We have characterized such bodies in actively growing and contact inhibited cells as to their acid phosphatase content, heavy metal content, autofluorescent characteristics, staining characteristics and morphology. It has been shown that many of these bodies are acid phosphatase positive, contain heavy metals, show the natural and characteristic fluorescence of lipofuscin pigment, are PAS-positive, acid fast with Ziehl-Neelsen and are morphologically similar to lipofuscin. They are thus analogous to the lipofuscin granules found in post-mitotic cells in vivo. The number of such granules was found to increase following contact inhibition of the mitotic activity. We conclude that lipofuscin granules are found continuously in the culture and that the cellular content of these granules can be diluted by cell division.
已显示在体外培养的处于活跃生长阶段的II期人神经胶质细胞中少量存在残留小体样结构。在接触抑制的II期神经胶质细胞中，这些结构会积累。这些结构是否类似于体内有丝分裂后细胞中的脂褐素色素颗粒尚不确定。我们已根据其酸性磷酸酶含量、重金属含量、自发荧光特性、染色特性和形态对活跃生长和接触抑制细胞中的此类小体进行了表征。已显示这些小体中的许多呈酸性磷酸酶阳性，含有重金属，呈现脂褐素色素的天然特征荧光，PAS阳性，齐-尼氏抗酸染色阳性，并且在形态上与脂褐素相似。因此，它们类似于体内有丝分裂后细胞中发现的脂褐素颗粒。发现此类颗粒的数量在有丝分裂活性受到接触抑制后会增加。我们得出结论，在培养物中持续发现脂褐素颗粒，并且这些颗粒的细胞含量可通过细胞分裂被稀释。
