Fermanian C, Lapeyre C, Fremy J M, Claisse M
Laboratoire Central d'Hygiène Alimentaire, Centre National d'Etudes Vétérinaires et Alimentaires, Maison-Alfort, France.
J Dairy Res. 1997 Nov;64(4):551-9. doi: 10.1017/s0022029997002379.
The growth of four Bacillus cereus strains producing diarrhoeal toxin at 32 degrees C (F4433/73 and 29.155, isolated on the occasion of foodborne outbreaks, and F4581/76L and F4581/76R, two variants of a clinical strain), a weakly toxigenic strain isolated in routine analysis of food (3505M) and an emetic isolate (F3502/73) was investigated at low temperature. Biomass was determined by protein assay. Generation times were: for strain F3502/73, which grew at > or = 12 degrees C, 8.71 h (at 12 degrees C); for other strains, which grew at > or = 10 degrees C, 10.2 to approximately 18.9 h (at 10 degrees C). Toxin production during growth was evaluated by a commercial kit (Oxoid) and by a toxicity test on Chinese hamster ovary cells. Strains F4433/73 and F4581/76, secreting high levels of diarrhoeal toxin during the exponential phase at 32 degrees C, produced high levels of toxicity at 10 degrees C until the stationary phase. Strain 29.155 had decreased toxin production at 10 degrees C. Toxicities for cellular extracts remained low when compared with culture filtrates. A correlation was found between the toxicity values given by the two detection methods tested, and the suitability of both methods for the detection of potential poisoning isolates is discussed.
在低温条件下研究了4株产生腹泻毒素的蜡样芽孢杆菌菌株（F4433/73和29.155，在食源性疾病暴发时分离得到；F4581/76L和F4581/76R，临床菌株的两个变体）、1株在食品常规分析中分离得到的弱产毒菌株（3505M）以及1株呕吐毒素分离株（F3502/73）的生长情况。通过蛋白质测定法测定生物量。代时分别为：F3502/73菌株在≥12℃时生长，代时为8.71小时（12℃时）；其他菌株在≥10℃时生长，代时为10.2至约18.9小时（10℃时）。通过商业试剂盒（Oxoid）以及对中国仓鼠卵巢细胞的毒性试验评估生长过程中的毒素产生情况。F4433/73和F4581/76菌株在32℃指数生长期分泌高水平腹泻毒素，在10℃时直至稳定期都产生高水平毒性。29.155菌株在10℃时毒素产生减少。与培养滤液相比，细胞提取物的毒性仍然较低。发现了两种检测方法给出的毒性值之间的相关性，并讨论了这两种方法用于检测潜在中毒分离株的适用性。
