Ikura K, Kodama S, Hashimoto H, Hayashi K, Mori H, Ichida M, Sorimachi M, Kudo K, Hara S
Kyoto Institute of Technology, Japan.
Biosci Biotechnol Biochem. 1997 Nov;61(11):1939-41. doi: 10.1271/bbb.61.1939.
Vibrio anguillarum is a pathogenic microorganism of vibriosis, an infectious disease found in various fish species. A mouse hybridoma clone, named C5, that produced a monoclonal antibody to V. anguillarum was established. The specific reaction of C5 antibody with V. anguillarum was confirmed by the pre-adsorption effect of the V. anguillarum cells in ELISA and a cell immunoprecipitation experiment. Western blotting analysis indicated that the C5 antibody recognized a high molecular weight substance extracted from cells with detergents.
鳗弧菌是弧菌病的病原微生物，弧菌病是在多种鱼类中发现的一种传染病。建立了一个名为C5的小鼠杂交瘤克隆，该克隆产生了一种针对鳗弧菌的单克隆抗体。通过ELISA中鳗弧菌细胞的预吸附作用和细胞免疫沉淀实验，证实了C5抗体与鳗弧菌的特异性反应。蛋白质印迹分析表明，C5抗体识别用去污剂从细胞中提取的一种高分子量物质。
