Yoshioka M, Shimizu C, Harwood F L, Coutts R D, Amiel D
Department of Orthopaedics, University of California San Diego, La Jolla 92093-0630, USA.
Osteoarthritis Cartilage. 1997 Jul;5(4):251-60. doi: 10.1016/s1063-4584(97)80021-0.
Ninety-nine mature New Zealand White (NZW) rabbits underwent unilateral anterior cruciate ligament transection (ACLT) and were divided into three groups. The contralateral non-operated knees served as controls. The first group (SA) received intra-articular injections of 0.3 ml hyaluronan (HA: MW; 8 x 10(5)) beginning 4 weeks after ACLT, once a week for 5 weeks. The second group (SV) was injected with vehicle (carrier of HA) in the same fashion as the SA group. The third group (SN) served as a nontreatment group post ACLT. All animals were killed 9 weeks post-surgery and were assessed by gross morphology, histomorphometry and biochemical analysis. Gross morphologic changes on the femoral cartilage in the SA group were less severe than those in the SV and SN groups. Cartilage thickness, cartilage area, and thickness of synovial lining cell layer histomorphometric parameters were measured, showing a positive effect of HA on the preservation of articular cartilage and synovial tissue. Similarly, the cartilage and synovial tissues from knees injected with HA did not demonstrate significant alterations from contralateral controls as measured by biochemical analysis [i.e., water content, pyridinoline concentration, glycosaminoglycan (GAG) content for the cartilage, and DNA concentration for the synovial tissue].
99只成年新西兰白兔接受单侧前交叉韧带切断术（ACLT），并分为三组。对侧未手术的膝关节作为对照。第一组（SA）在ACLT术后4周开始每周进行一次关节内注射0.3 ml透明质酸（HA：分子量；8×10⁵），共注射5周。第二组（SV）以与SA组相同的方式注射赋形剂（HA的载体）。第三组（SN）作为ACLT术后的非治疗组。所有动物在术后9周处死，并通过大体形态学、组织形态计量学和生化分析进行评估。SA组股骨软骨的大体形态学变化比SV组和SN组轻。测量了软骨厚度、软骨面积和滑膜衬里细胞层厚度等组织形态计量学参数，显示HA对关节软骨和滑膜组织的保存有积极作用。同样，通过生化分析（即软骨的含水量、吡啶啉浓度、糖胺聚糖（GAG）含量以及滑膜组织的DNA浓度）测量，注射HA的膝关节的软骨和滑膜组织与对侧对照相比没有显示出明显变化。
