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采用两端改性羟基磷灰石的丝胶原支架重建兔前交叉韧带：组织学和生物力学研究。

Anterior cruciate ligament reconstruction in a rabbit model using a silk-collagen scaffold modified by hydroxyapatite at both ends: a histological and biomechanical study.

机构信息

Department of Orthopedic Surgery, The First Affiliated Hospital of Zhengzhou University, NO.1 Jianshe East Road, Zhengzhou, 450001, China.

Henan University of Chinese Medicine, NO.156 Jinshui East Road, Zhengzhou, 450001, China.

出版信息

J Orthop Surg Res. 2021 Feb 16;16(1):139. doi: 10.1186/s13018-021-02281-0.

DOI:10.1186/s13018-021-02281-0
PMID:33593365
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7885370/
Abstract

BACKGROUND

To investigate osteointegration at the graft-bone interface and the prevention of osteoarthritis after anterior cruciate ligament (ACL) reconstruction using a silk-collagen scaffold with both ends modified by hydroxyapatite (HA) in a rabbit model.

METHODS

The HA/silk-collagen scaffold was fabricated using a degummed, knitted silk scaffold, collagen I matrix, and simulated body fluid (SBF). The HA/silk-collagen scaffold was rolled up to make a graft for replacing the native ACL in the experimental group (HA group), and the silk-collagen scaffold was used in the control (S group). All specimens were harvested at 16 weeks postoperatively to evaluate graft-bone healing and osteoarthritis prevention.

RESULTS

Histological staining revealed the massive formation of more mature bone at the tendon-bone interface, and immunohistochemistry staining revealed more collagen I and osteocalcin deposition in the HA group than in the S group. Higher signals indicating more bone mineral formation were detected in the HA group than in the S group, which was consistent with the results of biomechanical testing. Better osteoarthritis prevention was also observed in the HA group, indicating a more stable knee joint in the HA group than in the S group.

CONCLUSION

The HA/silk-collagen scaffold promotes osteointegration at the tendon-bone interface after ACL reconstruction and has great potential for clinical applications.

摘要

背景

为了在兔模型中研究 ACL 重建后使用两端经羟基磷灰石（HA）修饰的丝胶原支架的移植物-骨界面的骨整合和预防骨关节炎。

方法

使用脱胶、针织丝支架、I 型胶原基质和模拟体液（SBF）制备 HA/丝胶原支架。将 HA/丝胶原支架卷起制成替代天然 ACL 的移植物用于实验组（HA 组），而丝胶原支架用于对照组（S 组）。所有标本均在术后 16 周收获，以评估移植物-骨愈合和预防骨关节炎。

结果

组织学染色显示在肌腱-骨界面大量形成更成熟的骨，免疫组织化学染色显示在 HA 组中比在 S 组中沉积更多的 I 型胶原和骨钙素。在 HA 组中检测到更多的骨矿物质形成的信号，表明更多的骨形成，这与生物力学测试的结果一致。在 HA 组中也观察到更好的骨关节炎预防，表明 HA 组的膝关节比 S 组更稳定。

结论

HA/丝胶原支架促进 ACL 重建后肌腱-骨界面的骨整合，具有很大的临床应用潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/8c787344fbfc/13018_2021_2281_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/349456e9cbba/13018_2021_2281_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/52f05baee64d/13018_2021_2281_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/751d7dc84970/13018_2021_2281_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/641be9db10cb/13018_2021_2281_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/da81b6fd6158/13018_2021_2281_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/4a2e71653b3f/13018_2021_2281_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/92f5b6dcad8d/13018_2021_2281_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/8c787344fbfc/13018_2021_2281_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/349456e9cbba/13018_2021_2281_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/52f05baee64d/13018_2021_2281_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/751d7dc84970/13018_2021_2281_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/641be9db10cb/13018_2021_2281_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/da81b6fd6158/13018_2021_2281_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/4a2e71653b3f/13018_2021_2281_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/92f5b6dcad8d/13018_2021_2281_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fea8/7885370/8c787344fbfc/13018_2021_2281_Fig8_HTML.jpg

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