Caproni M, Bianchi B, D'Elios M M, De Carli M, Amedei A, Fabbri P
Department of Dermatology, University of Florence, Italy.
Allergy. 1997 Nov;52(11):1063-70. doi: 10.1111/j.1398-9995.1997.tb00177.x.
CD30 expression was evaluated by immunohistochemistry in lesional skin biopsies of eight patients with active atopic dermatitis (AD) and three patients with allergic contact (nickel-induced) dermatitis (ACD). CD30 expression was also assessed in a large panel of CD4+ and CD8+ T-cell clones generated from the skin biopsies of four patients with AD. Finally, the levels of soluble CD30 (sCD30) were measured in the serum of 41 patients with AD, 19 patients with ACD, and 60 healthy controls. In all specimens of lesional AD skin, where the great majority of infiltrating cells were CD4+ T cells, remarkable numbers of cells were CD30+, whereas virtually no CD30+ cells were found in the skin of patients with ACD. In CD4+ T-cell clones generated from the lesional AD skin, most of which produced both interleukin (IL)-4 and interferon-gamma (IFN-gamma) (Th0-like cells) or IL-4 and IL-5, but not IFN-gamma (Th2-like cells), CD30 expression directly correlated with the ability to produce IL-4 and IL-5, but was inversely related to IFN-gamma production. High levels of sCD30 (correlated with disease activity: r = 0.618) were detected in the serum of most AD patients, whereas there was no increase of sCD30 levels in the serum of patients with ACD. These data support the view that Th0/Th2-type responses predominate in the skin of patients with AD and suggest that the presence of CD30+ T cells in tissues and/or increased levels of sCD30 in biologic fluids are indicative of Th2-dominated responses.
采用免疫组织化学方法对8例活动性特应性皮炎（AD）患者和3例过敏性接触（镍诱导）性皮炎（ACD）患者的皮损活检组织进行CD30表达评估。还对从4例AD患者皮肤活检组织中产生的大量CD4⁺和CD8⁺T细胞克隆进行了CD30表达评估。最后，检测了41例AD患者、19例ACD患者和60例健康对照者血清中可溶性CD30（sCD30）水平。在所有AD皮损标本中，绝大多数浸润细胞为CD4⁺T细胞，有相当数量的细胞CD30⁺，而在ACD患者皮肤中几乎未发现CD30⁺细胞。在从AD皮损皮肤产生的CD4⁺T细胞克隆中，大多数产生白细胞介素（IL）-4和干扰素-γ（IFN-γ）（类似Th0细胞）或IL-4和IL-5，但不产生IFN-γ（类似Th2细胞），CD30表达与产生IL-4和IL-5的能力直接相关，但与IFN-γ产生呈负相关。大多数AD患者血清中检测到高水平的sCD30（与疾病活动相关：r = 0.618），而ACD患者血清中sCD30水平未升高。这些数据支持以下观点，即Th0/Th2型反应在AD患者皮肤中占主导地位，并表明组织中CD30⁺T细胞的存在和/或生物体液中sCD30水平的升高表明以Th2为主导的反应。
