Korohoda W, Madeja Z
Department of Cell Biology, J. Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Biochem Cell Biol. 1997;75(3):263-76.
The shape and locomotion of rat sarcoma XC cells on glass, polystyrene, and confluent monolayer cultures of aligned human skin fibroblasts were studied with quantitative, computer-assisted methods. The cell shape depended upon the substratum; the sarcoma cells seeded on fibroblasts assumed polarized shapes. The tumour cells emigrating from aggregates and in sparse cultures showed random locomotion when plated on glass or on the polystyrene surface of tissue culture dishes in isotropic conditions. However, when sarcoma cell aggregates were plated onto underlying aligned fibroblasts, the sarcoma cells showed contact guidance, migrating along the long axes of fibroblasts. Simultaneously, suppression of migration normal to the axis of fibroblasts orientation was observed. The sarcoma cells displaced a few times faster on aligned fibroblasts than under isotropic conditions in control cultures. This fast displacement was found to result from the less frequent cell turnings and straightening of cell trajectories (i.e., from klinokinesis), and not from an acceleration of cell movement and the longer cell tracks (i.e., not from orthokinesis). The presented results support the suggestion of Abercrombie (M. Abercrombie. 1979. Nature (London). 281: 259-262.) that tumour cells may be guided by the underlying normal cells when invading surrounding tissues and forming metastases.
采用定量计算机辅助方法，研究了大鼠肉瘤XC细胞在玻璃、聚苯乙烯以及人皮肤成纤维细胞排列的汇合单层培养物上的形态和运动。细胞形态取决于基质；接种在成纤维细胞上的肉瘤细胞呈现出极化形态。当在各向同性条件下接种在玻璃或组织培养皿的聚苯乙烯表面时，从聚集体中移出并处于稀疏培养状态的肿瘤细胞表现出随机运动。然而，当将肉瘤细胞聚集体接种到下层排列的成纤维细胞上时，肉瘤细胞表现出接触导向，沿着成纤维细胞的长轴迁移。同时，观察到垂直于成纤维细胞排列轴的迁移受到抑制。与对照培养物在各向同性条件下相比，肉瘤细胞在排列的成纤维细胞上的移动速度快几倍。发现这种快速移动是由于细胞转向频率降低和细胞轨迹变直（即由于运动性减弱），而不是由于细胞运动加速和细胞轨迹变长（即不是由于正向运动）。本文结果支持了Abercrombie（M. Abercrombie. 1979. Nature (London). 281: 259 - 262.）的观点，即肿瘤细胞在侵入周围组织并形成转移时可能受到下层正常细胞的引导。
