Abercrombie M, Turner A A
Med Biol. 1978 Dec;56(6):299-303.
An investigation of the behaviour of cells of the mouse transplantable sarcoma S180 in culture by time-lapse cinematography has been made to determine how the spreading of a population from an explant is affected by contact reactions between the sarcoma cells; and how the invasion of a fibroblast population is affected by contact reactions with the fibroblasts. Although the sarcoma cells show a form of contact inhibition to each other, this has little influence on the spreading of a population, which is brought about very largely by diffusive movement, in sharp contrast to the spreading of a fibroblast population. The invasion of a fibroblast population by S180 cells takes place as a consequence of defective contact inhibition; though contact inhibition (but not contact paralysis) of the S180 cells has an appreciable incidence. During invasion the S180 cells, though moving on the exposed surfaces of the fibroblasts, may be largely using the collagen substratum between the fibroblasts for their locomotion. The arrangement of the fibroblasts produces some degree of contact guidance of the sarcoma cells.
通过延时摄影术对小鼠可移植肉瘤S180细胞在培养中的行为进行了研究，以确定肉瘤细胞之间的接触反应如何影响从外植体扩散的细胞群体；以及成纤维细胞群体的侵袭如何受到与成纤维细胞接触反应的影响。尽管肉瘤细胞彼此之间表现出一种接触抑制形式，但这对细胞群体的扩散影响很小，细胞群体的扩散在很大程度上是由扩散运动引起的，这与成纤维细胞群体的扩散形成鲜明对比。S180细胞对成纤维细胞群体的侵袭是接触抑制缺陷的结果；尽管S180细胞的接触抑制（但不是接触麻痹）有相当高的发生率。在侵袭过程中，S180细胞虽然在成纤维细胞的暴露表面上移动，但可能在很大程度上利用成纤维细胞之间的胶原基质进行移动。成纤维细胞的排列对肉瘤细胞产生了一定程度的接触导向作用。
