RNA聚合酶II启动子解链和起始过程中的多个ATP依赖步骤。
Multiple ATP-dependent steps in RNA polymerase II promoter melting and initiation.
作者信息
Yan M, Gralla J D
机构信息
Department of Chemistry and Biochemistry, and Molecular Biology Institute, University of California, Los Angeles, CA 90095-1569, USA.
出版信息
EMBO J. 1997 Dec 15;16(24):7457-67. doi: 10.1093/emboj/16.24.7457.
Abstract
Permanganate probing and abortive initiation assays were used to investigate the role of ATP in several successive stages of transcription initiation at the activated adeno E4 and mouse DHFR promoters. Removal of ATP at several points along the multi-step pathway blocked further progress towards its completion. Most strikingly, even if the DNA transcription start site is opened using ATP, the subsequent removal of ATP disallows formation of the first phosphodiester bond of the RNA. After ATP-dependent formation of a short RNA, a new transcription complex forms, which is more stable and has a longer open region. Both RNA and ATP appear to play roles in the formation of this complex. The need for ATP throughout this multi-step initiation pathway leads to new and unexpected possibilities for the use of energy and ATPases in transcription initiation.
摘要
使用高锰酸盐探测和流产起始分析来研究ATP在活化的腺病毒E4和小鼠二氢叶酸还原酶（DHFR）启动子转录起始的几个连续阶段中的作用。在多步骤途径的几个点去除ATP会阻碍其进一步完成。最引人注目的是，即使使用ATP打开DNA转录起始位点，随后去除ATP也会阻止RNA第一个磷酸二酯键的形成。在依赖ATP形成短RNA后，会形成一个新的转录复合物，它更稳定且具有更长的开放区域。RNA和ATP似乎都在这个复合物的形成中发挥作用。在这个多步骤起始途径中对ATP的需求为转录起始中能量和ATP酶的使用带来了新的、意想不到的可能性。
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