过氧亚硝酸盐能迅速穿透磷脂膜。
Peroxynitrite rapidly permeates phospholipid membranes.
作者信息
Marla S S, Lee J, Groves J T
机构信息
Department of Chemistry, Princeton University, Princeton, NJ 08544, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14243-8. doi: 10.1073/pnas.94.26.14243.
Abstract
Peroxynitrite (ONOO-) is a potent oxidant implicated in a number of pathophysiological processes. The activity of ONOO- is related to its accessibility to biological targets before its spontaneous decomposition (t1/2 approximately 1 s at pH 7.4, 37 degrees C). Using model phospholipid vesicular systems and manganese porphyrins as reporter molecules, we demonstrated that ONOO- freely crosses phospholipid membranes. The calculated permeability coefficient for ONOO- is approximately 8.0 x 10(-4) cm.s-1, which compares well with that of water and is approximately 400 times greater than that of superoxide. We suggest that ONOO- is a significant biological effector molecule not only because of its reactivity but also because of its high diffusibility.
摘要
过氧亚硝酸根离子（ONOO-）是一种强效氧化剂，与多种病理生理过程有关。ONOO-的活性与其在自发分解之前对生物靶点的可及性有关（在pH 7.4、37℃时半衰期约为1秒）。使用模型磷脂囊泡系统和锰卟啉作为报告分子，我们证明ONOO-可自由穿过磷脂膜。计算得出的ONOO-渗透系数约为8.0×10-4 cm·s-1，与水的渗透系数相当，约为超氧阴离子渗透系数的400倍。我们认为ONOO-不仅因其反应活性，还因其高扩散性，是一种重要的生物效应分子。
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本文引用的文献
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2
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Chem Biol. 1995 Jul;2(7):437-45. doi: 10.1016/1074-5521(95)90259-7.
3
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J Biol Chem. 1997 Feb 7;272(6):3465-70. doi: 10.1074/jbc.272.6.3465.
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