对蛋白质稳定性至关重要的降解性共价反应。
Degradative covalent reactions important to protein stability.
作者信息
Volkin D B, Mach H, Middaugh C R
机构信息
Merck Research Laboratories, West Point, PA 19486, USA.
出版信息
Mol Biotechnol. 1997 Oct;8(2):105-22. doi: 10.1007/BF02752255.
PMID:9406181
Abstract
Commonly observed chemical modifications that occur in proteins during their in vitro purification, storage, and handling are discussed. Covalent modifications described include deamidation and isoaspartate formation, cleavage of peptide bonds at aspartic acid residues, cystine destruction and thiol-disulfide interchange, oxidation of cysteine and methionine residues, and the glycation and carbamylation of amino groups.
摘要
讨论了蛋白质在体外纯化、储存和处理过程中常见的化学修饰。所描述的共价修饰包括脱酰胺作用和异天冬氨酸形成、天冬氨酸残基处肽键的断裂、胱氨酸破坏和硫醇-二硫键互换、半胱氨酸和甲硫氨酸残基的氧化，以及氨基的糖基化和氨甲酰化。
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