Dubin D T, Shine J
Nucleic Acids Res. 1976 May;3(5):1225-31. doi: 10.1093/nar/3.5.1225.
We have examined the 3'-terminal sequence of the "small" structural ribosomal RNA ("13S") of hamster cell mitochondria, using a procedure involving [3H]isoniazide labeling of samples subjected to sequential periodate oxidation and beta-elimination. The terminus was found to be PyUAUUAOH, which is similar, but not identical, to the corresponding terminus of eukaryotic cytoplasmic 18S rRNA.
我们采用了一种方法,对经高碘酸盐连续氧化和β-消除处理的样品进行[3H]异烟肼标记,从而检测了仓鼠细胞线粒体“小”核糖体结构RNA(“13S”)的3'末端序列。发现其末端为PyUAUUAOH,这与真核细胞质18S rRNA的相应末端相似,但并不相同。