Weber M, Johannisson R, Bolte M, Ngo T K, Heller K H
Orthopaedic University Hospital, RWTH-Aachen, Germany.
Biotech Histochem. 1997 Sep;72(5):243-8. doi: 10.3109/10520299709082247.
A procedure is presented for exact, detailed comparison of light and electron microscopic analyses of tissues with complex architecture. Earlier techniques require one to make drawings of tissue pieces to be analyzed by electron microscopy to permit rough localization of the origin of the tissue pieces. Specifically, exact analysis of fetal cartilage and bone is hampered by the complicated arrangement of both tissue components, severely limiting the assessment of electron microscopic analyses. The advantage of the technique described here is that it allows precise localization of the tissue sample in the original tissue area. Punches 1 min in diameter were obtained from femora and coxae with a syringe and embedded for light and electron microscopy. The remaining tissue with its exactly defined punctures is prepared for standard histology. Human fetal cartilage and bone tissue were used to demonstrate this technique, but this procedure may be used for other kinds of tissues.
本文介绍了一种对具有复杂结构的组织进行光镜和电镜分析精确、详细比较的方法。早期技术要求人们绘制待用电镜分析的组织切片图，以便大致定位组织切片的来源。具体而言，胎儿软骨和骨骼的精确分析因两种组织成分的复杂排列而受到阻碍，严重限制了电镜分析的评估。这里描述的技术的优点是它允许在原始组织区域中精确定位组织样本。用注射器从股骨和髋骨获取直径1毫米的穿孔，并进行包埋用于光镜和电镜检查。其余带有精确界定穿孔的组织则用于标准组织学检查。使用人类胎儿软骨和骨组织来演示该技术，但此程序也可用于其他类型的组织。
