Weber M, Johannisson R, Rehder H, Graf J
Stiftung Orthopädische Universitätsklinik Heidelberg.
Z Orthop Ihre Grenzgeb. 1995 Sep-Oct;133(5):405-10. doi: 10.1055/s-2008-1039946.
A new technique is presented for the analysis of tissues which allows an exact, detailed comparison of light and electron microscopy. For this purpose, the previous techniques required the cutting of tissue pieces from the original tissue compound and the preparation of drawings as a rough localisation of the tissue pieces removed for analysis. Due to the processes of cutting and drawing, only an approximate localisation of the tissue investigated by electron microscopy was practicable. Specifically, the exact analysis of fetal cartilage-bone-tissue was mostly not possible due to the complicate arrangement of both tissue components. Therefore, the assessment of electron microscopic analyses was severely limited. The new technique, presented in this study, is characterised by a removal of punches 1 mm in diameter from the original tissue for electron microscopy. The remaining tissue with its exact defined punch-punctures is prepared for standard histology. Thus, the specific advantage of this new method is the precise attribution of the punched tissue to the original tissue group. Human fetal cartilage-bone-tissue has been used to demonstrate this punch-technique and the specific light- and electron microscopic preparations.
本文介绍了一种用于组织分析的新技术，该技术能够对光学显微镜和电子显微镜观察结果进行精确、详细的对比。为此，以往的技术需要从原始组织复合物中切取组织块，并绘制示意图作为对切除用于分析的组织块的大致定位。由于切割和绘图过程，通过电子显微镜研究的组织只能进行大致定位。具体而言，由于两种组织成分的复杂排列，对胎儿软骨-骨组织进行精确分析大多是不可能的。因此，电子显微镜分析的评估受到严重限制。本研究中提出的新技术的特点是从原始组织中取出直径为1毫米的穿孔用于电子显微镜观察。将剩余带有精确界定穿孔的组织准备用于标准组织学检查。因此，这种新方法的独特优势在于能够将穿孔组织精确归属于原始组织组。已使用人类胎儿软骨-骨组织来展示这种穿孔技术以及特定的光学显微镜和电子显微镜标本制备方法。
