Podhaisky H P, Abate A, Polte T, Oberle S, Schröder H
Department of Pharmacology and Toxicology, School of Pharmacy, Martin Luther University, Halle (Saale), Germany.
FEBS Lett. 1997 Nov 17;417(3):349-51. doi: 10.1016/s0014-5793(97)01307-0.
A 24-h incubation with hydrogen peroxide (0.65 mM) markedly reduced viability of cultured endothelial cells. Preincubation with aspirin (3-30 microM) protected endothelial cells from hydrogen peroxide-induced toxicity and increased viability in a concentration-dependent fashion by up to 64% of control. A similar protection was observed with D-alpha-tocopherol acetate (vitamin E, 3-30 microM). The cytoprotective effects of aspirin and vitamin E against hydrogen peroxide were overadditive suggesting different mechanisms of antioxidant action. In agreement with this, cytotoxicity induced by iron, the main catalyst of oxygen radical formation, was substantially reduced by aspirin but not vitamin E. These results show that aspirin protects endothelial cells from oxidative stress possibly via binding or chelation of free cytosolic iron. Moreover, a combination of aspirin and vitamin E might be useful for the prevention of endothelial injury in cardiovascular disease, e.g. during atherogenesis.
用0.65 mM过氧化氢孵育24小时显著降低了培养的内皮细胞的活力。用阿司匹林（3 - 30 microM）预孵育可保护内皮细胞免受过氧化氢诱导的毒性，并以浓度依赖的方式提高活力，最高可达对照的64%。用醋酸D-α-生育酚（维生素E，3 - 30 microM）也观察到类似的保护作用。阿司匹林和维生素E对过氧化氢的细胞保护作用是超相加的，提示抗氧化作用机制不同。与此一致的是，氧自由基形成的主要催化剂铁诱导的细胞毒性被阿司匹林显著降低，但维生素E没有。这些结果表明，阿司匹林可能通过结合或螯合游离的胞质铁来保护内皮细胞免受氧化应激。此外，阿司匹林和维生素E的组合可能有助于预防心血管疾病中的内皮损伤，例如在动脉粥样硬化形成过程中。
