P 因子诱导的重组体在雄性黑腹果蝇生殖系中的积累。
The accumulation of P-element-induced recombinants in the germline of male Drosophila melanogaster.
作者信息
Tanaka M M, Liang X M, Gray Y H, Sved J A
机构信息
School of Biological Sciences, Sydney University, NSW, Australia.
出版信息
Genetics. 1997 Dec;147(4):1769-82. doi: 10.1093/genetics/147.4.1769.
P-element-induced recombination in Drosophila melanogaster occurs premeiotically. Recombinants are therefore expected to accumulate in the stem cells of the germline of P-element-carrying males. We show that both the recombination frequency and the incidence of "clustering" increase with the age of males carrying various P-element derivatives. The combination of end-deleted elements can lead to average recombination frequencies >50% with individual instances of 100% recombination. These elements also lowered the fertility of the carriers. We investigated these features by constructing an analytical and a computer simulation model of the course of events in the germline, incorporating the recently proposed hybrid element insertion (HEI) model of P-element activity. The model is able to predict extreme recombination levels, segregation ratio biases and lowered fertility through cell death in a single analysis.
P 因子诱导的黑腹果蝇重组发生在减数分裂前。因此，重组体预计会在携带 P 因子的雄性果蝇生殖系的干细胞中积累。我们发现，携带各种 P 因子衍生物的雄性果蝇，其重组频率和“聚集”发生率均随年龄增长而增加。末端缺失元件的组合可导致平均重组频率>50%，个别情况下重组率可达 100%。这些元件还降低了携带者的生育能力。我们通过构建一个分析模型和一个计算机模拟模型来研究这些特征，该模型纳入了最近提出的 P 因子活性的杂交元件插入（HEI）模型。该模型能够在单一分析中通过细胞死亡预测极端的重组水平、分离比偏差和生育能力降低。
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