体外修饰的P因子在黑腹果蝇中诱导的种系和体细胞重组。
Germ-line and somatic recombination induced by in vitro modified P elements in Drosophila melanogaster.
作者信息
Sved J A, Eggleston W B, Engels W R
机构信息
School of Biological Sciences, Sydney University, New South Wales, Australia.
出版信息
Genetics. 1990 Feb;124(2):331-7. doi: 10.1093/genetics/124.2.331.
Abstract
The P element insertion delta 2-3(99B) has previously been shown to activate incomplete P elements elsewhere in the genome. We show that this element, in conjunction with a second incomplete P element, P[CaSpeR], also induces recombination in the male germ line. The recombination is induced preferentially in the region of the P[CaSpeR] element. Recombinant chromosomes contain the P[CaSpeR] element in more than 50% of cases, and alternative models of transposon replication and preferential chromosome breakage are put forward to explain this finding. As is the case with male recombination induced by P-M dysgenic crosses, recombination appears to be premeiotic in a high proportion of cases. The delta 2-3(99B) element is known to act in somatic cells. Correspondingly, we show that the delta 2-3(99B)-P[CaSpeR] combination elevates the incidence of somatic recombination.
摘要
P 因子插入δ2-3(99B)先前已被证明可激活基因组其他位置的不完整 P 因子。我们发现，该因子与第二个不完整 P 因子 P[CaSpeR]一起，也能在雄性生殖系中诱导重组。这种重组优先在 P[CaSpeR]因子区域诱导产生。在超过 50%的情况下，重组染色体含有 P[CaSpeR]因子，我们提出了转座子复制和优先染色体断裂的替代模型来解释这一发现。与 P-M 杂种不育杂交诱导的雄性重组情况一样，在很大比例的情况下，重组似乎发生在减数分裂前。已知δ2-3(99B)因子在体细胞中起作用。相应地，我们表明δ2-3(99B)-P[CaSpeR]组合提高了体细胞重组的发生率。
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