Máttar S, Mora A, Bernal N
Departamento de Microbiología, Facultad de Ciencias, Universidad Javeriana, Bogotá, D.C.
Enferm Infecc Microbiol Clin. 1997 Aug-Sep;15(7):364-8.
The aim of this study was to determine the prevalence of E. coli O157:H7 in pediatric patients with acute gastroenteritis (AGE).
A prospective epidemiologic study was performed in cases and controls to detect the germ in fecal samples of 300 children with AGE in Bogotá, D.C. The stools of the patients and 85 controls were seeded in MacConkey-sorbitol agar. The sorbitol negative colonies were biotyped and seroagglutinated with a latex test for E. coli O157:H7. Antimicrobial susceptibility was performed after using the Bauer & Kirby method.
Of the 300 diarrhea specimens analyzed, 14 strains corresponded to E. coli O157:H7 with a prevalence rate of 4.7% in children with AGE. The prevalence was 1.14%, the excess of risk of presenting E. coli O157:H7 was 14% in children with AGE. One child developed SUH. In three of the 85 controls E. coli O157:H7 was isolated, with a prevalence rate of 3.53%. The mean age of the 14 patients was 21 months (range: 3 months to 7 years) and the mean length of diarrhea in the children was 2 days. All the isolations of E. coli O157:H7 were sorbitol negative and indol negative with only 2 strains being MUG negative.
This epidemiologic study has, for the first time, demonstrated the strong prevalence of E. coli O157:H7 in Colombia. The clinical data do not show a common pattern. Whether the strains isolated in the study are verotoxin producers remains to be demonstrated.
本研究旨在确定急性胃肠炎（AGE）儿科患者中大肠杆菌O157:H7的流行情况。
在病例组和对照组中进行了一项前瞻性流行病学研究，以检测哥伦比亚特区波哥大300例AGE儿童粪便样本中的病菌。将患者和85名对照的粪便接种于麦康凯 - 山梨醇琼脂培养基上。对山梨醇阴性菌落进行生物分型，并通过大肠杆菌O157:H7乳胶试验进行血清凝集试验。采用鲍尔与柯比法进行药敏试验。
在分析的300份腹泻标本中，有14株对应大肠杆菌O157:H7，AGE儿童中的流行率为4.7%。流行率为1.14%，AGE儿童出现大肠杆菌O157:H7的额外风险为14%。一名儿童发生了溶血尿毒综合征（SUH）。在85名对照中有3例分离出大肠杆菌O157:H7，流行率为3.53%。14例患者的平均年龄为21个月（范围：3个月至7岁），儿童腹泻的平均时长为2天。所有分离出的大肠杆菌O157:H7均为山梨醇阴性和吲哚阴性，只有2株为4-甲基伞形酮基-β-D-葡萄糖醛酸苷（MUG）阴性。
这项流行病学研究首次证明了大肠杆菌O157:H7在哥伦比亚的高流行率。临床数据未显示出常见模式。研究中分离出的菌株是否产生志贺毒素仍有待证实。
