Lodato P B, Forchiassin F, Segovia de Huergo M B
Departamento de Ingeniería Química, Facultad de Ingeniería, Universidad de Buenos Aires, Argentina.
Rev Argent Microbiol. 1997 Jul-Sep;29(3):115-21.
Amylase production by a strain of Aureobasidium pullulans isolated in the laboratory was evaluated in liquid media (complex and synthetic) and in solid medium (wheat bran). There was an inhibitory effect in amylase production or amylase secretion by glucose. Asparagine was the best nitrogen source for amylase production (4-6 g/l). Only chlamidospores and melanin but not, amylase activity, were obtained with ammonium sulfate. Amylase production in solid culture was higher than the production obtained in the liquid media assayed. Optimum initial moisture content in solid culture ranged between 57 and 74%. No difference was observed in amylase production between solid media inoculated with cells grown in liquid or solid media.
对实验室分离的一株出芽短梗霉在液体培养基（复合培养基和合成培养基）和固体培养基（麦麸）中淀粉酶的产生情况进行了评估。葡萄糖对淀粉酶的产生或分泌具有抑制作用。天冬酰胺是淀粉酶产生的最佳氮源（4 - 6克/升）。使用硫酸铵时，仅获得了厚垣孢子和黑色素，未获得淀粉酶活性。固体培养中淀粉酶的产量高于所检测的液体培养基中的产量。固体培养的最佳初始水分含量在57%至74%之间。接种在液体或固体培养基中生长的细胞的固体培养基之间，淀粉酶产量未观察到差异。
