Golod E A
Urol Nefrol (Mosk). 1997 Sep-Oct(5):5-9.
The author studied the effects of 30-min heat ischemia of rat kidneys on the level of malonic dialdehyde (MDA) and Ca-dependent ATPase activity of microsomal fraction isolated from the cortical substance in the presence and absence of antibiotic alameticine and ortovandate in the incubation medium and protective action on Ca-ATPase activity of rat pretreatment with alpha-tocopherol (TP). It has been demonstrated that thermal ischemia induces inhibition of Ca-ATPase activity of microsomes resistant to vanadate. Administration of TP reduced MDA level, enhanced Ca-ATPase microsomal activity in the presence of alameticine against inhibition of enzymic activity in the absence of alameticine. This indicates a rise in the true enzyme activity under decreasing membrane permeability in conditions of diminishing activity of lipid peroxidation in response to TP effects.
作者研究了大鼠肾脏30分钟热缺血对从皮质物质分离的微粒体部分丙二醛（MDA）水平和钙依赖性ATP酶活性的影响，该研究在孵育介质中存在和不存在抗生素阿拉米辛和奥托万酸盐的情况下进行，还研究了α-生育酚（TP）预处理大鼠对Ca-ATP酶活性的保护作用。结果表明，热缺血诱导对钒酸盐耐药的微粒体Ca-ATP酶活性受到抑制。给予TP可降低MDA水平，在存在阿拉米辛的情况下增强微粒体Ca-ATP酶活性，以对抗在不存在阿拉米辛时酶活性的抑制。这表明在TP作用下，脂质过氧化活性降低，膜通透性降低，真正的酶活性增加。
