Iargin V N, Pylaev A S
Tsitologiia. 1976 Apr;18(4):464-9.
Cytophotometric, interferometric and autoradiographic methods were applied to evaluate hyperchromic motoneurons (HM) from spinal cord of mice which were allowed to swim for 4 hours. The HM in comparison with the normochromic motor nerve cells are characterized by a higher RNA content and a lower dry weight of perikaria. The autoradiographic data with H3-uridane make it possible to suggest that RNA exchange between the nucleus and cytoplasm of HM is accelerated. The mode of distribution of labelled proteins along the sciatic nerve 6 days after the local injection of H3-leucine in the ventral horn of L3--L4 spinal cord segments allows to conclude that the amount of protein transported with the slow component of axonal flow in the cases connected with the increase of the number of HM in corresponding nuclei is changed.
应用细胞光度法、干涉测量法和放射自显影法，对经4小时游泳的小鼠脊髓中的深染运动神经元（HM）进行评估。与正常染色的运动神经细胞相比，HM的特征在于RNA含量较高，而核周体的干重较低。用H3-尿苷进行的放射自显影数据表明，HM的细胞核与细胞质之间的RNA交换加速。在L3-L4脊髓节段腹角局部注射H3-亮氨酸6天后，标记蛋白沿坐骨神经的分布方式表明，在与相应核中HM数量增加相关的情况下，通过轴浆流慢成分运输的蛋白量发生了变化。
