Sacco M G, Zecca L, Bagnasco L, Chiesa G, Parolini C, Bromley P, Catò E M, Roncucci R, Clerici L A, Vezzoni P
Institute of Advanced Biomedical Technologies, National Research Council, Milan, Italy.
Nat Biotechnol. 1997 Dec;15(13):1392-7. doi: 10.1038/nbt1297-1392.
Transgenic mice for genotoxicity testing have been developed, although no such models have been produced for the evaluation of toxic, nongenotoxic chemical compounds. We have developed a transgenic mouse model for the analysis of toxic inorganic compounds. We engineered a mouse lineage with the human growth hormone (hGH) gene under the control of the human hsp70 promoter, in which a plasma-detectable hGH response can be elicited by exposure to heat shock. In primary cell cultures from these mice, hGH release was observed following treatment with several toxic inorganics. Transgenic mice injected intraperitoneally with sodium arsenite, cadmium chloride, copper sulphate, or methylmercurium chloride showed significant hGH levels in plasma.
用于遗传毒性测试的转基因小鼠已经培育出来了，不过尚未培育出用于评估有毒非遗传毒性化合物的此类模型。我们已经开发出一种用于分析有毒无机化合物的转基因小鼠模型。我们构建了一个小鼠品系，其人类生长激素（hGH）基因受人类hsp70启动子控制，在该品系中，暴露于热休克可引发血浆中可检测到的hGH反应。在这些小鼠的原代细胞培养物中，用几种有毒无机物处理后观察到了hGH释放。经腹腔注射亚砷酸钠、氯化镉、硫酸铜或氯化甲基汞的转基因小鼠血浆中显示出显著的hGH水平。
