酵母ARS1起始位点处DNA合成的离散起始位点。

Discrete start sites for DNA synthesis in the yeast ARS1 origin.

作者信息

Bielinsky A K, Gerbi S A

机构信息

Department of Molecular Biology, Cell Biology and Biochemistry, Division of Biology and Medicine, Brown University, Providence, RI 02912, USA.

出版信息

Science. 1998 Jan 2;279(5347):95-8. doi: 10.1126/science.279.5347.95.

DOI:10.1126/science.279.5347.95

PMID:9417033

Abstract

Sites of DNA synthesis initiation have been detected at the nucleotide level in a yeast origin of bidirectional replication with the use of replication initiation point mapping. The ARS1 origin of Saccharomyces cerevisiae showed a transition from discontinuous to continuous DNA synthesis in an 18-base pair region (nucleotides 828 to 845) from within element B1 toward B2, adjacent to the binding site for the origin recognition complex, the putative initiator protein.

摘要

利用复制起始点定位技术，已在酵母双向复制起点的核苷酸水平上检测到DNA合成起始位点。酿酒酵母的ARS1起点在元件B1内部至B2的一个18碱基对区域（核苷酸828至845）显示出从间断性到连续性DNA合成的转变，该区域邻近起点识别复合物（一种假定的起始蛋白）的结合位点。