Lemini C, Silva G, Timossi C, Luque D, Valverde A, González-Martínez M, Hernández A, Rubio-Póo C, Chávez Lara B, Valenzuela F
Departamento de Farmacología, Facultad de Medicina, UNAM, Ciudad Universitaria, Mexico.
Environ Res. 1997 Nov;75(2):130-4. doi: 10.1006/enrs.1997.3782.
Xenobiotic estrogens in the environment or diet have received much attention as a possible source of certain hormonal disease states in human and wildlife. Therefore, the detection of estrogenic activity of any substance, especially those related to the food industry, is important. The estrogenic activity of p-hydroxybenzoic acid (PHBA), a compound related to a commonly used group of preservatives in food, cosmetic, and pharmaceutical preparations, was evaluated with immature and adult ovariectomized female mice (CD1) using two well-known bioassays. Subcutaneous administrations (s.c.) of different doses of PHBA were compared with estradiol (E2), and their effects on vaginal cornification and uterotrophic activities were evaluated. Different groups of animals were treated s.c. daily for 3 days with vehicle (corn oil, 0.3 ml/100 g), E2 (1 microgram/100 g), and PHBA (0.5, 5, 50, and 500 micrograms/100 g). Four days after treatment, PHBA produced a dose-dependent response on vaginal cornification and uterotrophic activity in both immature and adult ovariectomized mice. The relative uterotrophic potency of PHBA (500 micrograms/100 g) to E2 (1 microgram/100 g) was 0.0011 in immature mice and 0.0018 in ovariectomized animals.
环境或饮食中的外源性雌激素作为人类和野生动物某些激素疾病状态的可能来源已受到广泛关注。因此，检测任何物质，尤其是与食品工业相关物质的雌激素活性非常重要。对羟基苯甲酸（PHBA）是一种与食品、化妆品和药物制剂中常用的一类防腐剂相关的化合物，使用两种著名的生物测定方法对未成熟和成年去卵巢雌性小鼠（CD1）评估其雌激素活性。将不同剂量的PHBA皮下注射（s.c.）与雌二醇（E2）进行比较，并评估它们对阴道角质化和子宫营养活性的影响。不同组的动物每天皮下注射给药3天，分别给予赋形剂（玉米油，0.3 ml/100 g）、E2（1微克/100 g）和PHBA（0.5、5、50和500微克/100 g）。治疗4天后，PHBA在未成熟和成年去卵巢小鼠中均对阴道角质化和子宫营养活性产生剂量依赖性反应。在未成熟小鼠中，PHBA（500微克/100 g）相对于E2（1微克/100 g）的相对子宫营养效力为0.0011，在去卵巢动物中为0.0018。
