Dai Jing, Zhang Linyuan, Xu Jianfeng, Peng Fangda, Wu Zhijun, Fu Longlong, Guo Ying, Chang Bing, Lu Wenhong, Ding Chunguang
National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing 102308, China.
National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Toxics. 2023 Jan 29;11(2):131. doi: 10.3390/toxics11020131.
Parabens are endocrine-disrupting chemicals (EDCs) that have estrogen-like activities and may cause male reproductive disorders. Here, we developed a method for the simultaneous determination of four parabens (MeP, EtP, n-PrP, n-BuP) and two metabolites (4-HB and 3,4-DHB) in human seminal plasma by UPLC-MS/MS. The method was used to analyze 144 seminal plasma samples from Chinese males. MeP, EtP, n-PrP, and 4-HB were the dominant compounds. MeP, EtP, and n-PrP were significantly correlated to each other. In addition, 4-HB was significantly correlated to MeP, EtP, n-PrP, and 3,4-DHB, respectively. The results provide direct evidence that parabens and their metabolites are widely distributed in the male reproductive system. The study presents the paraben metabolites levels in human seminal plasma for the first time.
对羟基苯甲酸酯是具有雌激素样活性的内分泌干扰化学物质（EDC），可能导致男性生殖系统紊乱。在此，我们开发了一种通过超高效液相色谱-串联质谱法（UPLC-MS/MS）同时测定人类精浆中四种对羟基苯甲酸酯（甲基对羟基苯甲酸酯、乙基对羟基苯甲酸酯、正丙基对羟基苯甲酸酯、正丁基对羟基苯甲酸酯）和两种代谢物（4-羟基苯甲酸、3,4-二羟基苯甲酸）的方法。该方法用于分析144份中国男性的精浆样本。甲基对羟基苯甲酸酯、乙基对羟基苯甲酸酯、正丙基对羟基苯甲酸酯和4-羟基苯甲酸是主要化合物。甲基对羟基苯甲酸酯、乙基对羟基苯甲酸酯和正丙基对羟基苯甲酸酯之间显著相关。此外，4-羟基苯甲酸分别与甲基对羟基苯甲酸酯、乙基对羟基苯甲酸酯、正丙基对羟基苯甲酸酯和3,4-二羟基苯甲酸显著相关。这些结果提供了直接证据，表明对羟基苯甲酸酯及其代谢物在男性生殖系统中广泛分布。该研究首次呈现了人类精浆中对羟基苯甲酸酯代谢物的水平。
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