de Conto Cinier C, Petit-Ramel M, Faure R, Garin D
Chimie Analytique 2, LICAS, Bâtiment 305, Physiologie Générale et Comparée, Bâtiment 404, Université Claude Bernard Lyon 1, 43 boulevard du 11 Novembre 1918, Villeurbanne Cedex, 69622, France.
Ecotoxicol Environ Saf. 1997 Nov;38(2):137-43. doi: 10.1006/eesa.1997.1569.
This work was an attempt to investigate cadmium bioaccumulation in the carp (Cyprinus carpio) during simulated pollution. The fish, weighing 100 g, were kept for 140 days in a 1000-liter indoor concrete tank supplied with a continuous flow (8 liters min-1) of unchlorinated, aerated, and filtered well water, the cadmium concentration of which was maintained at 450 microg liter-1. Such a high cadmium concentration was chosen to achieve metal saturation of the fish organs. Carp were fed during exposure. Cadmium accumulation in liver, kidney, and muscle was measured by inductively coupled plasma-mass spectrometry (ICP-MS), which is one of the most sensitive analytical techniques. The reported data indicate that cadmium exposure results in significant cadmium uptake, but the pattern of this uptake varies with the organ. For kidney and liver, cadmium concentration increased rapidly to the saturation level, probably because of the limited ability to store the cadmium as exposure persisted. A positive correlation between the increase in metallothioneins in tissues and the increase in metal tolerance in fish has been suggested. The cadmium concentration increase in muscle was significant only after 3 months, and, then, increased as an exponential function of the exposure time. When the storage capacity limits of the liver and kidney are reached, cadmium accumulation in muscle is stimulated. In this experiment, the high contamination levels reached suggest that such muscle would be unsuitable for human consumption. After 140 days of exposure, the cadmium concentrations in muscle, liver, and kidney were respectively 9 +/- 1, 91 +/- 7, and 250 +/- 16 mg kg-1 dry wt.
这项工作旨在研究模拟污染过程中鲤鱼（Cyprinus carpio）体内镉的生物累积情况。将体重100克的鱼置于一个1000升的室内混凝土水箱中，持续供应（8升/分钟）未加氯、曝气且经过过滤的井水，其中镉浓度维持在450微克/升。选择如此高的镉浓度是为了使鱼的器官达到金属饱和状态。在暴露期间给鲤鱼喂食。通过电感耦合等离子体质谱法（ICP-MS）测量肝脏、肾脏和肌肉中的镉累积量，这是最灵敏的分析技术之一。报告的数据表明，镉暴露会导致显著的镉摄取，但摄取模式因器官而异。对于肾脏和肝脏，镉浓度迅速上升至饱和水平，这可能是因为随着暴露持续，储存镉的能力有限。有人提出组织中金属硫蛋白的增加与鱼的金属耐受性增加之间存在正相关。肌肉中的镉浓度仅在3个月后才显著增加，然后作为暴露时间的指数函数增加。当肝脏和肾脏的储存能力达到极限时，肌肉中的镉累积就会受到刺激。在本实验中，达到的高污染水平表明这种肌肉不适合人类食用。暴露140天后，肌肉、肝脏和肾脏中的镉浓度分别为9±1、91±7和250±16毫克/千克干重。
