Ho Y K, Clifford A J
Department of Biology, City College of San Francisco, CA 94112, USA.
Comp Biochem Physiol B Biochem Mol Biol. 1997 Sep;118(1):167-72. doi: 10.1016/s0305-0491(97)00054-0.
A single generation of Drosophila melanogaster was raised on different media. One of the media was unsupplemented (the control) and the others were supplemented with pyrimidine analog at 10.3 mmol/kg culture medium. The relative numbers of larvae, pupae, and F1 adults reproduced from parent flies on each medium served as an indication of the relative toxicity of the supplements. The relative decreasing order of toxicity of the pyrimidines was as follows: 5-bromouracil < thymine < uracil = orotic acid = control = cytosine, control < UMP. The toxic effects of 5-bromouracil and thymine seem to be associated with the addition of a bromine or methyl group to carbon 5 of the pyrimidine ring. The UMP supplementation increased the number of adult F1 flies above the control group indicating that UMP was not only non toxic but also that it was beneficial.
让一代黑腹果蝇在不同培养基上生长。其中一种培养基不添加任何物质（作为对照），其他培养基则添加了10.3 mmol/kg培养基的嘧啶类似物。在每种培养基上，由亲代果蝇繁殖出的幼虫、蛹和F1代成虫的相对数量，可作为这些添加物相对毒性的指标。嘧啶的毒性相对递减顺序如下：5-溴尿嘧啶＜胸腺嘧啶＜尿嘧啶 = 乳清酸 = 对照 = 胞嘧啶，对照＜尿苷一磷酸。5-溴尿嘧啶和胸腺嘧啶的毒性作用似乎与嘧啶环第5位碳原子上添加溴或甲基有关。添加尿苷一磷酸使F1代成虫数量超过对照组，这表明尿苷一磷酸不仅无毒，而且有益。
