Renström A, Gordon S, Larsson P H, Tee R D, Newman Taylor A J, Malmberg P
Department of Occupational Health, National Institute for Working Life, Solna, Sweden.
Clin Exp Allergy. 1997 Nov;27(11):1314-21.
Mouse and rat urinary proteins are potent occupational allergens for exposed personnel. Methods of measuring airborne allergens differ greatly, and reported levels of allergens vary considerably between laboratories.
To compare the values obtained using two different methods of allergen detection.
Air samples were collected in rat rooms in Sweden and the United Kingdom at 2 L/min on to polytetrafluoroethylene (PTFE) filters and extracted in buffer containing 0.5% v/v Tween 20. Airborne rat urinary allergen (RUA) was measured in all samples by both RAST inhibition using a polyclonal human serum pool (UK) and a two monoclonal antibody sandwich ELISA employing antibodies specific for Rat n 1.02 (alpha2u-globulin) (Sweden).
The two methods gave values which were correlated (r2 log values = 0.72, P<0.0001), but differed by several orders of magnitude (median [range] ratio of RAST inhibition/ELISA = 316 [7-26(80)]. There was a systematic bias: as the absolute values increased, the difference in the measurements increased. The rat urine standards used were antigenically similar.
A large contrast in RUA values obtained from the two assays was observed in this study. This may be primarily due to methodological differences, but variations in antibody specificities or composition of allergenic epitopes in the air samples may contribute. The results demonstrate that standardization of methods and antibodies is necessary before interlaboratory comparisons can be made.
小鼠和大鼠的尿液蛋白是接触人员的强效职业过敏原。测量空气中过敏原的方法差异很大，各实验室报告的过敏原水平也有很大差异。
比较使用两种不同过敏原检测方法获得的值。
在瑞典和英国的大鼠饲养室中，以2升/分钟的流速将空气样本采集到聚四氟乙烯（PTFE）滤膜上，并在含有0.5%（v/v）吐温20的缓冲液中进行提取。所有样本均采用多克隆人血清库进行RAST抑制试验（英国）以及采用针对大鼠n 1.02（α2u球蛋白）的特异性抗体的双单克隆抗体夹心ELISA法（瑞典）来测量空气中的大鼠尿液过敏原（RUA）。
两种方法得到的值具有相关性（r2对数值 = 0.72，P<0.0001），但相差几个数量级（RAST抑制试验/ELISA的中位数[范围]比值 = 316 [7 - 26(80)]）。存在系统性偏差：随着绝对值增加，测量值的差异也增加。所使用的大鼠尿液标准品在抗原性上相似。
本研究观察到两种检测方法获得的RUA值存在很大差异。这可能主要是由于方法学差异，但空气样本中抗体特异性或过敏原表位组成的变化也可能有影响。结果表明，在进行实验室间比较之前，方法和抗体的标准化是必要的。
